C57BL/6細胞

年限： embryo

品系： C57/BL6

數量： 大量

運輸方式： 凍存運輸

ATCC Number： SCRC-1002?

器官來源： 胚胎

細胞類型： 胚胎干細胞

細胞形態： 球形

是否是腫瘤細胞： 0

物種來源： 小鼠

生長狀態： 貼壁生長

Designations: C57BL/6

Depositors: JC Conover, B Knowles

C57BL/6細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus deposited as mouse

Morphology: spherical colony

Source: Organ: embryo

Strain: C57/BL6

Cell Type: embryonic stem cell;

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Applications: Coat-color chimera production was high using c2J blastocysts while FVB blastocysts produced a low number of chimeras [PubMed: 11730008].

C57BL/6細胞The clonal embryonic stem cell line #693 ES C57BL/6 was derived from a strain C57BL/6J (B6) mouse blastocyst [PubMed: 11730008].

The ES cells were shown to populate the germ line of two host blastocyst donors, FVB/NJ (FVB) and the coisogenic strain C57BL/6-Tyrc-2J (c2J).

Age: embryo

Comments: The clonal embryonic stem cell line #693 ES C57BL/6 was derived from a strain C57BL/6J (B6) mouse blastocyst [PubMed: 11730008]. The ES cells were shown to populate the germ line of two host blastocyst donors, FVB/NJ (FVB) and the coisogenic strain C57BL/6-Tyrc-2J (c2J). Coat-color chimera production was high using c2J blastocysts while FVB blastocysts produced a low number of chimeras [PubMed: 11730008].

Propagation: ATCC complete growth medium: Mouse ES Cell Basal Medium

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Establishing and maintaining your culture:

To insure the highest level of viability, be sure to warm media to 37°C before using it on the cells.

Plate mitotically arrested MEF (CF-1) (ATCC SCRC-1040)as a feeder layer at least one day before plating the cells (see product sheet for mitotically arrested MEF for protocol). One hour before thawing the vial of cells, perform a 100% medium change using 4 mL of complete growth medium for ES cells C57BL/6細胞(see ATCC complete growth medium for recipe).

Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 90 seconds).

Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.

Transfer the vial s contents plus 5 mL of complete growth medium for ES cells to a 15 mL centrifuge tube. Use an additional 1 mL of media to rinse the vial and transfer the liquid to the 15 mL tube. Add 4 mL of complete growth medium for ES cells to bring the total volume to 10 mL.

Spin the cells at 270 xg for 5 min. Aspirate the supernatant and resuspend the pellet in 5 mL of complete growth medium for ES cells.

Add the 5 mL of cell suspension to the T75 flask containing feeder cells and 10 mL complete growth medium for ES cells.

Incubate the culture at 37?C in a humidified 5% CO2/95% air incubator. Perform a 100% medium change every day, passage cells every 1 to 2 days.

Interval: Every one to two days

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:7 is recommended

Medium Renewal: Every day

Preservation: Freeze medium: Complete growth medium supplemented with an additional 10% FBS and 10% DMSO.

Storage temperature: liquid nitrogen vapor phase

Related Products: recommended serum:ATCC SCRR-30-2020

Recommended medium: ATCC SCRR-2011

recommended serum: ATCC SCRR-30-2020

References: 16173597: C57BL/6細胞Brook FA, et al. The derivation of highly germline-competent embryonic stem cells containing NOD-derived genome. Diabetes. 52:205-208, 2003. PubMed: 12502514

16173598: Brook FA, Gardner RL. The origin and efficient derivation of embryonic stem cells in the mouse. Proc. Natl. Acad. Sci. USA. 94: 5709-5712, 1997. PubMed: 9159137